3M Attest Auto-reader 390 Quick Reference Guide

3M Quick Sterilization Assurance Reference Guide ™

For 3M™ Attest™ Auto-reader 390* and 3M™ Attest™ Rapid Readout Biological Indicators (BIs)**

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Cool

Allow test BI to cool at least 10 minutes.

Close

While wearing safety glasses, close the cap of the cooled BI by pushing the cap down.

Crush

Hold BI by cap and use “crusher well” to crush the media ampoule.

Tap

Holding BI by cap, tap the bottom of the vial on the counter until media wets spore strip.

Place

Place the activated BI in color-coded incubation well.

Interpret Result

Observe LCD panel under incubating BIs for negative (–) or positive (+) result. The positive control should read positive (+). A positive result for a test (processed) BI, which indicates a sterilization process failure, should be acted upon immediately.

Record

Record result for both test BI (processed) and positive control BI (unprocessed).

Optional Visual pH Color Change Result

Negative • Media remains purple. • A negative pH color change result can be assessed at 24 hours for Attest™ biological indicators 1291 and at 48 hours for Attest™ biological indicators 1292.

Positive • A media color change from purple to yellow indicates a positive result. • The biological indicator may be discarded as soon as the positive result has been observed and documented.

Infection Prevention Division 3M Health Care 2510 Conway Avenue St. Paul, MN 55144-1000 U.S.A. 1 800 228-3957 www.3M.com/infectionprevention

Please recycle. Printed in U.S.A. 3M and Attest are trademarks of 3M. © 3M 2013. All rights reserved. 70-2010-9397-1

* Refer to 3M™ Attest™ Auto-reader 390 Operator’s Manual for complete instructions ** Refer to 3M™ Attest™ Rapid Readout Biological Indicator (BI) Instructions for Use for complete instructions